Figure 2: Full-term development of phICSI-13 offspring.
From: Mice produced by mitotic reprogramming of sperm injected into haploid parthenogenotes
(a) Fluorescence images (top) showing DNA stained with Hoechst 33342 in 2-cell stage embryos (2C DNA), Hoffman images of blastocysts (blast) at embryonic day 5.0 (E5.0); pups and their associated placentae (post-natal day 1, P1) and adults with F1 offspring (adult/F1). In ICSI-1bla and phICSI-13-1bla, a single blastomere was destroyed (arrowheads) at the 2-cell stage. Scale bars, 30 μm. (b) Plots of body (step value, 0.01 g) and placenta (step value, 0.01 g) mass in ICSI- and phICSI-13-derived offspring (1- and 2-bla) at term (±s.d.). Average litter sizes are given in parentheses. For ICSI, n=19; ICSI-1bla, n=20; phICSI-13, n=21; phICSI-13-1bla, n=24. Experiments were on ≥4 days per group. Differences are shown for P<0.05 (1-way ANOVA followed by Tukey–Kramer test). (c) Kaplan–Meyer survival plots of ICSI- and phICSI-13-derived offspring (1- and 2-bla). For ICSI, n=20; ICSI-1bla, n=26; phICSI-13, n=19; phICSI-13-1bla, n=11. (d) Parthenogenotes ectopically expressing transgenic Plcz (PiV) were injected with B6D2F1 sperm (1C), showing DNA staining (blue) at the 2-cell stage (2C DNA), blastocyst (blast) development after 5 days (E5.0) and two male offspring and their associated placentae at term (P1). Scale bars, 50 μm.
