Figure 4: Male pronuclear transfer to haploid parthenogenotes.
From: Mice produced by mitotic reprogramming of sperm injected into haploid parthenogenotes
(a) Schematic representations of male pronuclear transfer into one blastomere of 2-cell haploid parthenogenotes (pnT-G1 and -G2, left). Schematic representations of control zygote cytoplast transfer into a 2-cell haploid parthenogenote blastomere (cytT-G1 and -G2, upper right) and pronuclear transfer into paternally-enucleated zygote (pnTz-G1 and -G2, lower right) are also shown. HVJ, hemagglutinating virus of Japan envelope vector. These controls determine in vivo developmental potential following manipulation at the 2-cell stage; data are presented in Supplementary Table 1. (b) Histograms showing in vitro developmental progress of pnT-G1 (open) and -G2 embryos (± s.e.m.). For pnT-G1, n=38 and pnT-G2, n=46, with experiments on 2 days. (c) Vertically paired images representative of in vitro development for pnT-G1 and -G2 E5.0 embryos and haploid parthenogenotes (1nP) showing Hoffman (bf, top) and paternally expressed membrane tdTomato (mtdT). Scale bar, 50 μm. (d) Paired fluorescence images of pnT-G1 embryos at the 2-cell stage showing DNA labelling (left) or treatment with BrdU (n=7). (e) Pups and associated placentae in pnT-G1 and the indicated control experiments, as they appeared after term delivery 19 d after embryo transfer (P0).
