Figure 6: Conserved and divergent histone modification in ICSI and phICSI-13.
From: Mice produced by mitotic reprogramming of sperm injected into haploid parthenogenotes
(a) Merged confocal fluorescence images showing DNA (stained with propidium iodide, red) and the indicated histone modifications (detected using antibodies, green) 2 h after sperm injection in ICSI (upper panels) or phICSI-13. The parental provenance of chromatin is indicated. The inset shows H3K9me2 staining in ph-13 parthenogenotes. Asterisks indicate modification differences. Scale bar, 20 μm. For ICSI H3K4me3, n=11; phICSI-13 H3K4me3, n=16; ICSI H3K9me2, n=20; phICSI-13 H3K9me2, n=24; ICSI H3K27me3, n=18; phICSI-13 H3K27me3, n=14; ICSI H4K12ac, n=18; phICSI-13 H4K12ac, n=16. (b) As for (a), except 6 h after sperm injection. For ICSI H3K4me3, n=9; phICSI-13 H3K4me3, n=10; ICSI H3K9me2, n=20; phICSI-13 H3K9me2, n=22; ICSI H3K27me3, n=12; phICSI-13 H3K27me3, n=22; ICSI H4K12ac, n=14; phICSI-13 H4K12ac, n=24. (c) Histograms showing H3K9me2 fluorescence quantification in ICSI and phICSI, indicating n for each group. These are, for ICSI 2 h, n=14; ICSI 6 h, n=15; phICSI-13 2 h, n=17; phICSI-13 6 h, n=14. Values (± s.e.m.) indicate differences where P<0.05 (two-tailed, unpaired t-test). Experiments were on 3 days.
