Figure 8: Distinctive ICSI and phICSI 2-cell embryo transcriptomes.
From: Mice produced by mitotic reprogramming of sperm injected into haploid parthenogenotes
(a) FDR-adjusted P-values for all 6,435 possible array assignments to the two equally-sized (8+8) ICSI groups (smoothed blue area) plotted over the gene rank according to smallest P-value. The red curve indicates the most extreme (largest number of adjusted P-values <0.05) combination that respects the experimental 2-cell sibling pairings. The inset shows the empirical density of P-values. Where there is no difference between ICSI and phICSI (true null hypothesis), a uniform density is expected. (b) FDR-adjusted P-values calculated for 10,000 out of 735,471 possible 16+8 ICSI-phICSI array groupings (smoothed blue area). The red curve represents the true ICSI-phICSI group assignment. (c) Normalized heatmap for the top 73 most differentially expressed genes (FDR P<0.05) between ICSI and phICSI. Values indicate different blastomeres. ICSI_1 and ICSI_2 refer to paired sibling cells in ICSI 2-cell embryos. (d) Classes of GO annotation terms obtained from the DAVID database. (e) Ratiometric qPCR analysis of pluripotency factor mRNAs in ICSI-1bla (n=5) and phICSI-13-1bla (n=8) morulae after 72 h. Values are±s.e.m. and differences indicated where P<0.05 (unpaired t-test).
