Figure 3: Ultraviolet-deprotection and analytical characterization of pCys peptide 6a and 6b. | Nature Communications

Figure 3: Ultraviolet-deprotection and analytical characterization of pCys peptide 6a and 6b.

From: Chemoselective synthesis and analysis of naturally occurring phosphorylated cysteine peptides

Figure 3

(a) Light-induced photolysis of peptide 4d to deliver pCys peptide 6a in 65% isolated yield. (b) UPLC-UV traces before (in blue) and directly after ultraviolet light exposure (in magenta). (c) 31P-NMR experiment showing the characteristic peak for pCys residues (12.02 p.p.m.) and inorganic phosphate. (d) EThcD MS/MS spectra of pCys peptide 6b, showing complete sequence coverage and diagnostic fragment ions c4, and z10. a.u., arbitrary unit.

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