Figure 4: Characterization of an endogenous pCys peptide by EThcD MS/MS.
From: Chemoselective synthesis and analysis of naturally occurring phosphorylated cysteine peptides
(a) Glucose-specific phosphoenolpyruvate-dependent phosphotransferase system. The phosphate group is transfered from PEP sequentially to the IIBGlc domain of the membrane protein IICBGlc. (b) SDS–PAGE in-gel trypsin digeston approach. (c) In solution tryptic digestion and offline two-dimensional LC approach.
