Figure 1: Biochemical characterization of the OPTN/TBK1 interaction. | Nature Communications

Figure 1: Biochemical characterization of the OPTN/TBK1 interaction.

From: Structural insights into the interaction and disease mechanism of neurodegenerative disease-associated optineurin and TBK1 proteins

Figure 1

(a) A schematic diagram showing the domain organizations of OPTN, TBK1 and NAP1. In this drawing, the OPTN/TBK1 and NAP1/TBK1 interactions are further highlighted and indicated by two-way arrows. (b) Co-IP assay to map the detailed interaction between TBK1 and OPTN, showing that the CTD (residues 677–729) of TBK1 and the N-terminal region (residues 1–119) of OPTN are responsible for their interaction. (c) Analytical gel filtration chromatography analyses of the interaction between TBK1 CTD and OPTN(26–119). (d) ITC-based measurement of the binding affinity of TBK1 CTD with the OPTN(26–119) fragment. The KD error is the fitted error obtained from the data analysis software, when using the one-site binding model to fit the ITC data. The ITC-measured N value, which is related to the binding stoichiometry, indicates that the binding stoichiometry of TBK1 CTD and OPTN(26–119) interaction is 1:1.

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