Figure 6: The two-subunit PaPC is required for growth on selected carbon sources.
From: A distinct holoenzyme organization for two-subunit pyruvate carboxylase
(a) Shaken liquid-culture growth of wild-type P. aeruginosa PA14, ΔPC, the PC-complemented strain and various site-specific mutants in a defined medium containing 20 mM succinate, pyruvate or glucose as the sole carbon source. Each growth curve represents the average of three biological replicates. Error bars denote s.d. (b) Pellicles formed by P. aeruginosa PA14, ΔPC, and the PC-complemented strain on a defined medium containing succinate, pyruvate, or glucose as the sole carbon source. (c) Colony morphology of P. aeruginosa PA14, ΔPC and the PC-complemented strain on a defined medium (supplemented with 1% agar, Congo red and Coomassie blue) containing succinate, pyruvate or glucose as the sole carbon source. Images depict day 3 of colony development.
