Figure 7: PaPC dysfunction leads to increased matrix production and altered colony morphology.
From: A distinct holoenzyme organization for two-subunit pyruvate carboxylase
(a) Colony morphology of wild-type P. aeruginosa PA14, ΔPC, the PC-complemented strain and the K572A mutant on 1% tryptone, 1% agar (supplemented with Congo red and Coomassie blue). (b) Quantification of Congo red binding, a proxy for matrix production, for P. aeruginosa PA14, ΔPC, the PC-complemented strain and various site-specific mutants grown on 1% tryptone, 1% agar with no added dyes. Colonies were grown for 3 days before they were collected for the Congo red binding assay. (c) Shaken liquid-culture growth of P. aeruginosa PA14, ΔPC, the PC-complemented strain and various site-specific mutants in 1% tryptone. Each growth curve represents the average of three biological replicates. Error bars denote s.d.
