Figure 1: Generation of DA neuron-specific FoxO1 KO (FoxO1 KO^DAT) mice.

(a) DA neuron-specific FoxO1 deletion. Green fluorescence indicates FoxO1. TdTomato (Td, red) was used to visualize DA neurons expressing DAT-cre. DAPI stains nuclei (blue). Scale bar, 50 μm. (b) Allele-specific PCR using different brain areas from FoxO1^F/+, FoxO1^F/F, and FoxO1 KO^DAT (DAT-cre; FoxO1^F/F). SN, substantia nigra. Hypo., hypothalamus. Cb, cerebellum. (c,d) Immunoblots (c) and relative FoxO1 protein levels (d) from indicated brain regions of FoxO1 KO^DAT and WT littermates. (e) Brain weight of FoxO1 KO^DAT and WT littermates. (f) Representative figures and graphs showing the brain width of FoxO1 KO^DAT and WT littermates. Scale bar, 10 mm. (g–i) Representative figures (g) and graphs showing the number (h) and size (i) of DA neurons of FoxO1 KO^DAT and WT littermates. Scale bar, 50 μm. The values are mean±s.e.m. (*P<0.05, **P<0.01, Student’s t-test).