Figure 6: Increased catecholamines level and iBAT thermogenesis in FoxO1 KODAT mice.
From: FoxO1 in dopaminergic neurons regulates energy homeostasis and targets tyrosine hydroxylase
(a) Serum norepinephrine levels of WT and KO male and female mice. (b) Dopamine contents measured from the midbrain and SN samples of WT and KO male mice. (c) Gene expression in iBAT of WT and KO male mice. (d) Immunoblots for p-Creb, p-p38 MAPK, UCP1, PGC1α in iBAT. (e) Relative protein levels for p-Creb, p-p38 MAPK, UCP1, PGC1α in iBAT from (d). Normalized to total p38 MAPK or GAPDH. (f) Representative figures of H&E staining and UCP1 staining from iBAT samples of WT and KO mice (n=3). Scale bar, 50 μm. (g) Quantification of mitochondrial DNA contents from iBAT samples of WT and KO mice. ♂, male. ♀, female. Data are expressed as mean±s.e.m. (*P<0.05, **P<0.01, Student’s t-test).
