Figure 2: Semi-quantitative replacement of KARs.

(a,b) Sample traces illustrating KAR currents evoked by glutamate uncaging on TEs (a) and soma (b) of CA3 PCs in organotypic slice cultures prepared from GluK2^+/+ and GluK2^−/− mice not transfected (NT) or transfected with different amounts of GluK2a cDNA (80, 20 and 3 ng μl⁻¹). (c,d) Bar graphs summarizing the average amplitude of KAR currents evoked by glutamate uncaging on TEs (c) or on the soma (d) in the different conditions (mean amplitude per cell). GluK2^+/+ NT: n=22; GluK2^−/− NT: n=11; 80 ng μl⁻¹: n=14; 40 ng μl⁻¹: n=6; 20 ng μl⁻¹: n=11; 10 ng μl⁻¹: n=12; 6 ng μl⁻¹: n=7; 3 ng μl⁻¹: n=16; 43 mice. (e,f) Sample traces of KAR currents evoked by glutamate uncaging shown in a and scaled at the peak illlustrating differences in the decay kinetics for various conditions. (f) Quantification of the decay rate (tau weighted) of KAR currents evoked by glutamate uncaging for the different conditions. GluK2^+/+ NT: n=7; 80 ng μl⁻¹: n=7; 40 ng μl⁻¹: n=6; 20 ng μl⁻¹: n=10; 10 ng μl⁻¹: n=12; 6 ng μl⁻¹: n=8; 3 ng μl⁻¹: n=6; 43 mice. In all panels, values are presented as mean ±s.e.m. of n experiments. Data were compared using one-way analysis of variance followed by Dunnett’s multiple comparison test (NS, not significant; P>0.05, **P<0.01 and ***P<0.001).