Figure 5: Subcellular distribution of SEP–GluK2a in CA3 PCs. | Nature Communications

Figure 5: Subcellular distribution of SEP–GluK2a in CA3 PCs.

From: Molecular determinants for the strictly compartmentalized expression of kainate receptors in CA3 pyramidal cells

Figure 5

(a) Sample images illustrating CA3 neurons electroporated with 3 ng μl−1 (top) or 80 ng μl−1 (bottom) SEP–GluK2 cDNA (green), TdTomato (blue) and live stained for the SEP moiety using anti-GFP nanobody-ATTO647 (red). Representative confocal z-stack projections are shown for each condition. Insets represent characteristic CA3 TEs or DDs. Arrows show the co-localized SEP and anti-GFP nanobody signals that correspond to SEP–GluK2 clusters. (b) Average number of clusters per unit length at the soma (S), TE or DD for each condition (3 ng μl−1: n=11 cells, 3 different experiments, 3 mice; 80 ng μl−1: n=10 cells, 3 different experiments, 3 mice). In all panels, values are presented as mean ±s.e.m. of n experiments. Data were compared using unpaired t-test (*P<0.05 and ****P<0.0001).

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