Figure 1: Transient Dam expression in PFC neurons.
From: Longitudinal assessment of neuronal 3D genomes in mouse prefrontal cortex
(a,b) Tissue sections from PFC harvested (a) 2 and (b) 10 days after HSV-TALEGad1Dam/mCherry injection. Notice robust mCherry (red) expression in neuronal layers at day 2 but not day 10 post injection. Section were immunolabelled for NeuN neuronal marker (green) and counterstained with 4,6-diamidino-2-phenylindole (blue). (a,b) Scale bars, 100 μm (left); 50 μm (upper right); 25 μm (lower right). (c) Representative example of bilateral HSV-TALEGad1Dam/GFP injection, showing robust transgene expression within ∼1 mm rostrocaudal strip of rostro-medial cortex. Microscopical image from Bregma+1.42 mm injection site, showing bilateral needle tracks and robust GFP expression in surrounding tissue. Scale bar, 1 mm. CC, corpus callosum; Cg, cingulate cortex; CPu, caudate putamen; Cx, cortex; M1, M2, motor cortex; PL (IL), pre-(infra-)limbic cortex. (d) Quantitative reverse transcriptase–PCR to quantify Dam and, for comparison, Gad1 and Gad2 RNA expression 2, 7 and 10 days after injection of HSV amplicon encoding TALEGad1Dam. Dam RNA, assayed with two independent primer pairs Dam.1 and Dam.2, expressed as fold change after normalization to 18S rRNA. N=4 per time point; data shown as mean±s.e.m. Notice highest Dam expression on day 2. *P=0.029, Mann–Whitney test (both Dam.1 and Dam.2).
