Figure 2: Direct interaction of IL-13Rα2 complexes and TMEM219 nanodisc.
From: IL-13Rα2 uses TMEM219 in chitinase 3-like-1-induced signalling and effector responses
(a,b) Serial dilution of TMEM219 nanodisc (10–10,000 nM) were incubated with 10 nM of BIOPYD-labelled Chi3l1 or IL-13Rα2. No significant increase of fluorescence signals was noted in these nanodisc incubations. (c) Serial dilution of TMEM219 nanodisc (10–10,000 nM) were incubated with 10 nM of BIOPYD-labelled IL-13Rα2-Chi3l1 complexes. As the concentration of the nanodisc increases, changes in fluorescence signal increases until it reaches saturation. IL-13Rα2-Chi3l1 complexes directly bind to TMEM219 nanodisc with an affinity of 288 nM, respectively. All the panels are representative of a minimum of two separate experiments.
