Figure 3: TMEM219 plays a critical role in Chi3l1-stimulated HB-EGF expression by epithelial cells and macrophages.
From: IL-13Rα2 uses TMEM219 in chitinase 3-like-1-induced signalling and effector responses
(a,b) HB-EGF production by 1HAEo cells. 1HAEo cells were treated rhChi3l1 (500 ng ml−1) with (+) and without (−) siRNA silencing of IL-13Rα2 or IL-13Rα1 or TMEM219. The concentrations of HB-EGF were assessed by ELISA. (c) HB-EGF production by macrophages. Peritoneal macrophages were prepared from WT, IL-13Rα2 or TMEM219 null mutant mice and incubated with rmChi3l1 (500 ng ml−1) for 48 h. The levels of supernatant HB-EGF were assessed by ELISA. (d) Effects of anti-TMEM219 on HB-EGF production by 1HAEo cells. 1HAEo cells were stimulated for 48 h with rhChi3l1 (500 ng ml−1) in the presence and absence of TMEM219 neutralizing antibodies. In all panels, the levels of supernatant HB-EGF were assessed by ELISA. The values represent the mean±s.e.m. of triplicate evaluations in a minimum of three separate experiments. **P<0.01; ***P<0.001; ns, non-significant by Student's t-test.
