Figure 8: Metazachlor alters TGN-associated SVs morphology and tubular interconnections at TGN. | Nature Communications

Figure 8: Metazachlor alters TGN-associated SVs morphology and tubular interconnections at TGN.

From: Enrichment of hydroxylated C24- and C26-acyl-chain sphingolipids mediates PIN2 apical sorting at trans-Golgi network subdomains

Figure 8

Transmission electron microscopy (TEM) of TGN membrane structures in Arabidopsis root treated (e–h) or not (a–d) with metazachlor. (a) Untreated cell showing Golgi apparatus (GA) and the SVs visible as a tubulo-vesiculated membrane network (SVs/TGN) at the trans-side of the Golgi. (e) Metazachlor-treated cell (50 nM) showing Golgi apparatus and swollen TGN-associated SVs. (b,f) Quantification show that the average diameter of SVs per TGN is around 80 nm in untreated cells (b), while being around 160 nm in metazachlor-treated cells (n=22 TGN for each for each experiment over 3 biological replicates, statistics were done by two-sided Welch’s two-sample t-test, ***P-value<0.001). (f,c,d) Magnification from a displaying tubular interconnections (black arrows) between SVs at TGN in untreated cells. (g,h) Magnification from e displaying larger SVs without tubular interconnections detected between them. Scale bars, 100 nm (a,e) and 50 nm (c,d,g,h).

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