Figure 2: Characterization of DSCAM-AS1.

(a) Plot highlighting the expression in FPKM of DSCAM-AS1 in the 6,503 sample MiTranscriptome RNA-seq compendium⁷ categorized by the different cancer/tissue types. Each point represents one RNA-seq tissue sample. (b) Expression of DSCAM-AS1 is significantly higher in ER-positive breast cancer tissue samples (n=584) compared with ER-negative samples (n=174). Expression was analysed in samples for which ER IHC was performed. Each point represents one RNA-seq sample. ***P<0.0001, comparing ER-positive with -negative. (c) Expression of DSCAM-AS1 by RNA-seq in breast cancer cell lines categorized by ER status. DSCAM-AS1 expression is significantly higher in ER-positive cell lines (n=21) versus ER-negative cell lines (n=29). Each point represents one cell line. ***P<0.0001, comparing ER-positive to –negative via Student’s t-test. (d) UCSC genome browser depiction of DSCAM-AS1 region on chromosome 21. RNA-seq expression track shown in red, and ER ChIP-seq shown in blue. Refseq transcripts shown in green. RACE verified transcript structure shown in black. (e) qPCR expression of DSCAM-AS1, GREB1, PGR, and MALAT1 8 h following addition of DMSO vehicle (black), 10 nM estrogen (red), and 10 nM estrogen and 1 μM tamoxifen (blue) in MCF7 and T47D cell lines. Error bars represent s.e.m. for three biological replicates. **P<0.001, ***P<0.0001, NS: P>0.01 comparing with vehicle for each condition via Student’s t-test. NS, not significant.