Figure 2: GDF11 treatment leads to bone loss in aged mice.

(a) Representative images of microCT reconstruction of distal femurs. Scale bar, 500 μm. (b) Von Kossa staining of undecalicified sections of femurs. Scale bar, 500 μm. (c) TRAP staining of femur sections from vehicle and rGDF11 treated mice. Scale bar, 50 μm. (d) Histomorphometric analysis of the metaphysis region of distal femurs. Results are shown as mean±s.d.; n=6; *P<0.05 by t test. (e) Representative images of lumber 4 vertebrae stained with Von Kossa. Scale bar, 500 μm. (f) Histomorphometric analysis of the trabecular bone in vertebrae. Results are shown as mean±s.d.; n=6; *P<0.05 and **P<0.01 by t test. (g,h) The serum levels of CTX and P1NP. Results are shown as mean±s.d.; n=6; **P<0.01 by t test.