Figure 2: BRCA2T2 binds to different DNA substrates and with stronger affinity than CTD.
From: A second DNA binding site in human BRCA2 promotes homologous recombination
(a) M size markers. (1) SDS–PAGE showing purified CTD (5 μg) loaded in a 4–15% acrylamide gel. (2) Western blot of the gel shown with an antibody against the His tag of the CTD and an antibody specific to DSS1 protein co-purified with it. (b) EMSA and quantification comparing the binding of BRCA2T2 and CTD to ssDNA (167mer). (c) dsDNA (40mer), (d) 3' ssDNA overhang, (e) 5' ssDNA overhang, (f) gapped DNA. Error bars, s.d. (n=3).
