Figure 4: BRCA2T2-BRC4 and BRCA2T2 stimulate RAD51-promoted DNA strand exchange.
From: A second DNA binding site in human BRCA2 promotes homologous recombination
(a) DNA strand exchange reaction using a 3' overhang ssDNA substrate (scheme on top) in the presence or absence of RPA, RAD51, and increasing concentrations of BRCA2BRC4-T2 or BRCA2. (b) Quantification of (a,c), same reaction as in a with increasing concentrations of BRCA2T2 or CTD. (d) Quantification of (c,e), same reaction as in a with increasing concentrations of BRCA2T2 or BRCA2T2 mutant in C315S, or C279A/C315S, as indicated. (f) Quantification of (e,g), same reaction as in a using a ssDNA substrate (scheme on top) with increasing concentrations of BRCA2BRC4-T2 or BRCA2T2 mutant C315S, as indicated. (h) Quantification of g. Error bars, s.d. (n=3).
