Figure 2: Mitochondrial engineering for isoprene production. | Nature Communications

Figure 2: Mitochondrial engineering for isoprene production.

From: Dual regulation of cytoplasmic and mitochondrial acetyl-CoA utilization for improved isoprene production in Saccharomyces cerevisiae

Figure 2

(a) Production of isoprene in recombinant strains. (b) Cell growth of recombinant strains. (c) Schematic diagram of a modified GAL regulation. (d) Two-stage process involved in the growth of GAL80-knockout strains. In the first stage, the genes under control of PGAL were expressed at a low level to sustain cell growth; while in the second stage, these genes were overexpressed at a high level. (e) Isoprene production and biomass of BY4742-M-04 MISPS-MISPS cultured in SG-URA (2% galactose), SD-URA (2% Dextrose) and SS-URA (2% Sucrose). The data in a,b,e are representative of three separate experiments. Bar represents mean±s.d.

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