Figure 3: Administration of RvD1 to WD-fed Ldlr−/− mice with established atherosclerosis restores lipid medicator balance.
(a) Quantification of lesional RvD1 and LTB4 levels (left and middle panels) and RvD1:LTB4 ratio (right panel) in aortic lesions of 17-week WD-fed Ldlr−/− mice that were administered vehicle control (Veh) or 100 ng RvD1/mouse (3 × /week i.p.) during weeks 12–17; the ratio data in 3a right panel are also shown for mice fed the WD for 8 weeks (Early lesions). For a, data are shown as mean±s.e.m., one-way ANOVA with Fisher’s least significant difference post-hoc analysis, *P<0.05, n=8 for early lesions, n=11 for advanced lesions/Veh group, and n=10 for advanced lesions/RvD1 group. (b) Representative images and quantification of lesional oxCaMKII by immunofluorescence microscopic analysis. MFI data are shown as mean±s.e.m., one-way ANOVA with Tukey’s multiple comparison test, *P<0.05 and ***P<0.001 early lesions versus other groups. ^P<0.05 Veh versus RvD1 groups, n=8 for early lesions, n=11 for advanced lesions/Veh group, and n=10 for advanced lesions/RvD1 group. Scale bar, 100 μm. (c) Representative confocal immunofluorescence images and quantification of 5-LOX localization in aortic lesional macrophages of 17-week WD-fed Ldlr−/− mice given vehicle control (Veh) or RvD1 during weeks 12–17. Macrophage F4/80 is red, 5-LOX is green, and Hoechst (nuclei) is blue. Arrows indicate nuclear region. In these images, nuclear 5-LOX appears as blotchy light blue staining within the dark blue Hoechst-stained nuclei. The quantitative data are shown as mean±s.e.m., t-test, *P<0.05, n=11 for Veh group and n=10 for RvD1 group. Scale bar, 5 μm.
