Figure 2: Substitution of 14 Bat NP-specific amino acids in CH4 is required to rescue SC35M.
From: A conserved influenza A virus nucleoprotein code controls specific viral genome packaging
(a) Alignment of SC35M NP and CH4 variants. Bat NP-specific amino acids are indicated in red. (b) The 19 Bat NP-specific amino acids in CH4 (highlighted in black) were mutated to SC35M-specific amino acids leaving either 14 (CH4.14), 4 (CH4.4) or no Bat NP-specific amino acids (CH4.0) and 100, 86 and 80 Bat NP-specific nucleotides, respectively. Relative polymerase activities of the indicated CH4 variants were determined by polymerase reconstitution assays in HEK293T cells. Mean and s.d. of three independent experiments are indicated in parenthesis. +successful rescue; −no rescue of recombinant viruses. (c) MDCKII cells were infected at an MOI of 0.001 with either wt SC35M, rCH4.4 or SC35M-CH4.0. At the indicated time points post infection (p.i.) virus titres were determined by plaque assay. Error bars indicate the mean and s.d. of at least three independent experiments.
