Figure 2: Hematopoietic-specific 14-3-3ζ deficiency results in defective arterial thrombosis. | Nature Communications

Figure 2: Hematopoietic-specific 14-3-3ζ deficiency results in defective arterial thrombosis.

From: 14-3-3ζ regulates the mitochondrial respiratory reserve linked to platelet phosphatidylserine exposure and procoagulant function

Figure 2

(a–d) Electrolytic injury was induced in the carotid artery of four cohorts of bone marrow-transplanted mice (refer to key): 14-3-3ζ-wt donor bone marrow (BM) into 14-3-3ζ-wt (n=9) or 14-3-3ζ-null (n=8) recipient mice and 14-3-3ζ-null donor BM into either 14-3-3ζ-wt (n=9) or 14-3-3ζ-null (n=7) recipients, as described in ‘Methods’. Carotid artery blood flow over 60 min (a), as well as total blood flow (b), time of vessel patency (c) and time to initial occlusion (d) were quantified over 60 min, as described in the legend to Fig. 1. Results represent mean±s.e.m. (*P<0.05), where results were analysed using a one-way ANOVA with Sidaks post hoc testing. (e) Haemostasis was examined using a 3 mm tail lop model of bleeding time, as described under ‘Methods’. Results are expressed as time taken to bleeding cessation, and represent the mean±s.e.m. (14-3-3ζ-wt, n=11; 14-3-3ζ-null, n=7). Results were analysed using an unpaired Student’s t-test (NSP>0.05).

Back to article page