Figure 8: 14-3-3ζ deficiency enhances platelet mitochondrial respiratory reserve capacity. | Nature Communications

Figure 8: 14-3-3ζ deficiency enhances platelet mitochondrial respiratory reserve capacity.

From: 14-3-3ζ regulates the mitochondrial respiratory reserve linked to platelet phosphatidylserine exposure and procoagulant function

Figure 8

Washed platelets were isolated from 14-3-3ζ-wt (black circles/bars) or 14-3-3ζ-deficient (14-3-3ζ-null, white circles/bars) mice. Mitochondrial respiration was measured in DMEM-modified media using the Seahorse XFp analyzer, according to the manufacturer’s instructions. (a) Representative trace identifying the typical pattern of OCR, depicting basal level, proton leak, ATP production, maximal respiration and reserve capacity, following inhibition of individual electron transport chain complexes (indicated by arrowheads), including: (1) vehicle or agonist; (2) oligomycin; (3) FCCP; and (4) rotenone/antimycin A. Platelets were assayed for oxygen consumption in unstimulated (b) or CRP/Thr-stimulated (0.25 μg ml−1/0.5 U ml−1) (c,d) conditions. (d) Reserve capacity in CRP/Thr-stimulated platelets was calculated based on the difference between the basal and maximal OCR (c). Results represent the mean±s.e.m. (n=8), and were analysed using two-way ANOVA (Bonferroni’s post hoc), where **P<0.01; *P<0.05.

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