Figure 2: Ubp2 and 3 mainly deubiquitinate Rsp5 substrates and have increased interactions with Rsp5 after HS. | Nature Communications

Figure 2: Ubp2 and 3 mainly deubiquitinate Rsp5 substrates and have increased interactions with Rsp5 after HS.

From: Deubiquitinase activity is required for the proteasomal degradation of misfolded cytosolic proteins upon heat-stress

Figure 2

(a,b) Increased ubiquitination levels after a 15 min HS at 45 °C in the indicated cells quantified by dot-blot and compared with unstressed cells (25 °C). Experiments were done with three biological replicates and averaged values are shown with s.d. In each experiment, increased ubiquitination levels were compared, as shown, to the deletion or WT strains using a two-tail student t-test (***P<0.001; **P<0.01; *P<0.05). a.u., arbitrary units. (c,d) Cells expressed 3xHA-tagged Rsp5 from a plasmid and endogenously C-terminally TAP-tagged UBP2 or UBP3, when indicated (black circles). Before lysis, cells that remained at 25 °C (unfilled circles) or heat shocked (40 °C, 20 min; black circles) were crosslinked for the remaining 10 min with 1% formaldehyde. Western blots of the indicated proteins are shown.

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