Figure 2: Integrative analysis of heart ChIP-seq data identifies >80,000 heart enhancers.

(a) Examples of heart enhancers identified through this analysis that had previously been validated in transgenic mouse assays²⁴. For each locus, we show the raw p300/CBP ChIP-seq signal from foetal human heart, the locations of the integrative analysis-identified enhancer and the tested element, and a representative E11.5 embryo. The heart is indicated by a red arrow. hs/mm numbers indicate the VISTA identifier. Scale bars, 1 kb. (b) Representative examples of putative enhancers with high, medium and low levels of support. Raw ChIP-seq data from all human heart samples analysed is shown, along with the corresponding confidence scores for each locus. (c) Histogram of combined scores for all 82,119 putative heart enhancers identified by the integrative analysis (left axis). Superimposed (red) is the retrospective heart enhancer validation rate versus combined score for all in vivo tested sites that overlap scored regions (see the ‘Methods’ section). (d) Cumulative proportion of in vivo-validated heart enhancers captured by score rank. Dashed gray line indicates the total number of validated heart enhancers considered (170). Rhombi indicate the total number of scored loci in each category.