Figure 4: H2A.Z modulates Lhb TSS and Cga +1 nucleosomes.
From: H2A.Z controls the stability and mobility of nucleosomes to regulate expression of the LH genes
(a) ChIP for H2A.Z was carried out in LβT2 cells, using the same sets of primers as in Fig. 1a,b. Data are analysed and presented as in Fig. 1d; n=4. (b) Typical unzipping curves for Lhb TSS (blue) and Cga +1 (red) nucleosomes reconstituted with H2A.Z, each with its corresponding naked DNA sequence (black). (c) Breaking force for region 1 and region 2 interactions for 601 (dashed green), Lhb TSS (dashed blue) and Cga +1 (dashed red) nucleosomes, reconstituted with H2A.Z. For comparison, the H2A nucleosome data from Fig. 2 are also shown (solid colours). Data shown as mean±s.e.m., n=27, 34 and 38; P=0.002, 0.02, 0.001, 0.002, 0.004 and 0.04, two-sample Kolmogorov–Smirnov test. *P<0.05, **P<0.01, ***P<0.001. (d) Two-dimensional scatter plots of region 2 breaking force versus position, for all the individual measured traces for H2A.Z reconstituted 601 (left, green), Lhb TSS (middle, blue) and Cga +1 (right, red) nucleosomes. The data for the same sequences reconstituted with H2A (Fig. 2f) are also shown in the background (grey). (e) Positional dispersion of the Lhb TSS and Cga +1 nucleosomes reconstituted with H2A.Z (dashed blue and dashed red, respectively), and for comparison nucleosomes on the same sequences but reconstituted with H2A. Data shown as region 2 position s.d.±s.e., n=34, 38; P=9 × 10−5, two-sample Ansari–Bradley test. ***P<0.001.
