Figure 1: ARHGAP36 interacts with PKAC. | Nature Communications

Figure 1: ARHGAP36 interacts with PKAC.

From: Bimodal antagonism of PKA signalling by ARHGAP36

Figure 1

(a) Schematic representation of human ARHGAP36 isoform 2 (UniProt ID: Q6ZRI8-2). (b) HEK293T cells were transfected with PKAC-YFP and Flag-ARHGAP36 or Flag-Cherry control. Lysates were immunoprecipitated using a Flag antibody and immunoblotted with GFP or Flag antibodies. Note the reduction of PKAC in presence of ARHGAP36. (c) HEK293T cells were transfected with YFP-ARHGAP36 or YFP-Cherry control. Lysates were immunoprecipitated using a GFP antibody and immunoblotted with GFP or PKAC antibodies. As in b, note the reduction of PKAC in the presence of ARHGAP36. (d) Confocal live micrographs of MDCK cells expressing CFP-ARHGAP36 and PKAC-YFP, either alone or together. Scale bars, 10μm. (e) FLIM–FRET measurements in MDCK cells expressing PKAC-YFP (donor) either alone, or together with mCherry-ARHGAP36 (acceptor) before and after acceptor photobleaching. Shown are YFP and mCherry confocal images and pseudocoloured donor fluorescence lifetime maps. Scale bars, 10μm. Right panel: corresponding histograms of the prebleach (red) and postbleach (blue) Venus-YFP lifetime values together with the donor only control sample (black).

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