Figure 7: Multidimensional profiles reveal mutant relationships and differences in the phenotypic spectrum.

(a) Hierarchical clustering suggests altered pathways. Highlights represent known genetic or phenotypic relationships. Mutants known to work in the same pathway, but with no visible phenotype, are accurately clustered together (jkk-1 and jnk-1). Mutations that cause mislocalization to dendritic domains are also clustered together, from their axonal, previously undetected, phenotypes alone (cdka-1, cdk-5 and cdka-1). Mutants with very similar phenotypes, and known to work in synaptic vesicle clustering (syd-2 and sad-1) also cluster together. Complementation test confirmed a175 is a new allele of lin-44. A list of all clustered genotypes in order appears in Supplementary Materials. (b) Network representing the average differences between populations computed from a weighted phenotypic profile. Subtle mutants isolated in this screen, imperceptible by visual inspection, are labelled in pink. Subtle mutants lie closest to wild type, while most previously identified mutants are the farthest from wild type.