Figure 4: ICER/CREM−/− mice are resistant to AIGN. | Nature Communications

Figure 4: ICER/CREM−/− mice are resistant to AIGN.

From: ICER is requisite for Th17 differentiation

Figure 4

Mice were immunized with rabbit IgG in complete Freund’s adjuvant (CFA) (day −3) and then injected intravenously with 200 μl of rabbit nephrotoxic serum (day 0). (a) Representative images of haematoxylin and eosin (left) and Periodic acid–Schiff (PAS) (right)-stained sections of kidneys collected by day 21 are shown. Scale bar, 50 μm. (b) Inflammation scores of kidney are shown (*P<0.05, ***P<0.001; mean±s.e.m.; two-way analysis of variance (ANOVA); n=4). (c) Glomerular injury was quantified by proteinuria (urinary albumin normalized to creatinine) at the indicated time points (P=0.0011; mean±s.e.m.; two-way repeated measures ANOVA; n=12). (df) Absolute cell numbers of (d) infiltrated CD4+ T cells, (e) IL-17A-producing CD4+ T cells and (f) IFNγ-producing CD4+ T cells in kidneys collected at day 21 were evaluated by flow cytometry (*P<0.05, **P<0.01; mean±s.e.m.; n=6).

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