Figure 5: ICER/CREM−/− mice are resistant to EAE. | Nature Communications

Figure 5: ICER/CREM−/− mice are resistant to EAE.

From: ICER is requisite for Th17 differentiation

Figure 5

EAE was induced in ICER/CREM+/+ and ICER/CREM−/− mice by immunization with MOG35–55 emulsified in CFA. (a) Spinal cord collected at day 14 from indicated mice were stained with haematoxylin and eosin (left) and luxol fast blue (right) to assess inflammation and myelin content, respectively. Scale bars, 500 or 100 μm (magnified panels). (b) Quantitative cumulative data are shown (*P<0.05; mean±s.e.m.; n=4). (c) The clinical scores and %body-weight changes were monitored (****P<0.0001; mean±s.e.m.; two-way analysis of variance (ANOVA); cumulative results of three independent experiments, n= 5, 5 and 6). (d–f) Absolute cell numbers of spinal cord-infiltrated (d) CD4+ T cells, (e) IL-17A-producing CD4+ T cells and (f) IFNγ-producing CD4+ T cells from indicated mice were evaluated by flow cytometry (n=8 per group). ICER/CREM+/+ mice without inducing EAE were used as a control (n=2) (*P<0.05; mean±s.e.m.; one-way ANOVA). (g,h) Mononuclear cells were collected at day 8 from inguinal lymph nodes and further cultured ex vivo with MOG for 3 days. (g) IL-17A/F and (h) IFNγ concentrations were measured by ELISA (*P<0.05; mean±s.e.m.; n=7).

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