Figure 4: NLRC4 inflammasome promotes angiogenesis in diet-induced obese mice.

(a) Left panel: proteome profiler array for angiogenesis proteins using tumour lysates from ND and HFD mice (red boxes indicate the internal reference spots). Right panel: analysis of proteome profiler. Data represent the fold change compared with ND of the average mean pixel density±s.d. of select proteins. (b) Representative immunohistochemistry (IHC) staining for CD31 (brown colour, some indicated with white arrows) from the indicated Py8119 tumours, haematoxylin (blue) being used for background nuclear staining. An isotype-negative control staining is included (negative control). Scale bar, 200 μm. (c) Quantification of IHC staining in b. Data represent the average area of CD31-positive staining over total area±s.d. At least three fields per section and three tumours per group were used in the analysis. (d) Data represent the average number of CD31⁺ cells as a percentage of total cells counted by flow cytometry±s.d. in E0771 from tumours of the indicated mice (n=5 all groups). (e,f) Average Vegfa mRNA expression relative to Ppia in Py8119 tumours (e) and E0771 tumours (f) from the indicated mice±s.d. (n=3 for each group). All studies represent results from two to three repeats. For all panels, the group means were compared by Student's t-test to determine significance.