Figure 5: Lys209 is a determinant for CYP98A9 flavonoid hydroxylase activity.
From: Evolutionary interplay between sister cytochrome P450 genes shapes plasticity in plant metabolism
(a) Docking of S-naringenin in the AtCYP98A9 active site. Arg96, Trp109 and Lys209 anchor naringenin for B-ring 3′-hydroxylation. (b) Lys209 substitution with alanine or isoleucine reduces AtCYP98A9 naringenin hydroxylase activity in vitro. Error bars represent the s.e. from three independent assays. (c) Fitmodel reconstitution of the history of the selection pressure on the AtCYP98A9 Lys209 codon. The most frequent codon and amino acid are indicated on branches.
