Figure 4: T1D patients present a humoural autoimmune response against IL-2.
(a) Percentage of anti-rhIL-2 positive subjects among five different cohorts: serum samples were obtained from healthy donors (HD, n=249) and patients diagnosed with type 2 (T2D; n=24) or type 1 diabetes (n=39 in cohort 1, n=15 in cohort 2 and n=21 in cohort 3). P-values were calculated using pairwise Fisher exact tests. (b) IgG subclass-specific anti-IL2 ELISA in T1D patients, absorbance OD measured at 450 after incubation of sera diluted 1:50. (c) T1D patient samples were divided into three groups based on EC50 values for an IL-2 direct ELISA from total sera (weak, moderate and strong binders) and are plotted relative to the anti-IL-2 EC50 value of IgG purified from the respective serum sample. Mean and s.d. overlay individual data points, and P-values were calculated using Welch’s t-test. (d) (upper panel) rhIL-2 competition ELISA using IgG purified from the sera of T1D patients, with the control (lower panel) of an anti-influenza ELISA with competing soluble rhIL-2.
