Figure 6: Correlation of MAN2C1 expression with loss of PTEN function in primary prostate cancer.
From: α-Mannosidase 2C1 attenuates PTEN function in prostate cancer cells
(a) Representative images of dual-IF (immunofluorescent) staining of prostate cancer tissues for PTEN (red) or MAN2C1 (green). Nuclei were counterstained with 4,6-diamidino-2-phenylindole (DAPI, blue). PTEN-positive (upper two panels) and -negative (lower panel) carcinomas in the same patient are shown. Scale bar represents 10 and 25 μm, respectively. Images were captured using a Zeiss LSM510 confocal microscrope and LSM510 Control Imaging software. (b) Hematoxylin and eosin (HE) and IHC staining of carcinoma (Ca), high-grade PIN and normal prostatic gland. All tissues were from the same patient/slide. Scale bar, 50 μm. (c) TMA2+TMA5 were IHC stained and analysed as a pooled population. Scores were grouped as negative (0) or positive (1). The number of cases expressing MAN2C1 (+ or −), AKT-P (+ or −) and PTEN (+ or −) are indicated on the top of individual bars.
