Figure 6: HiPSC-derived LRTM1⁺ cells survived and differentiated into mature DA neurons in primate PD model.

(a) HE staining of the grafts containing LRTM1⁺ cells at 12 weeks after transplantation. Scale bar, 500 μm. (b) Immunofluorescence images of the graft derived from day 28 LRTM1⁺ cells for SC-121 (blue), FOXA2 (green) and TH (red) at 12 weeks after transplantation. Scale bars, 500 μm. (c) Quantification of graft volume and TH⁺ cells in the grafts derived from day 21, day 28 and day 35 LRTM1⁺ cells at 12 weeks after transplantation (n=2 for each day). (d) DAB staining of a graft derived from day 28 LRTM1⁺ cells for TH at 12 weeks after transplantation. Scale bars, 200 μm. (e–j) Immunofluorescence images of a graft containing LRTM1⁺ cells for FOXA2 (green), NURR1 (green), PITX3 (green), DAT (green), GIRK2 (green), TH (red), KI67 (red) and 4′, 6′-diamidino-2-phenylindole (DAPI; blue) at 12 weeks after transplantation. Scale bars, 30 μm (e, f), 15 μm (g–i) and 50 μm (j).