Figure 2: Morphological and molecular alterations in the spinal cords of PSL mice. | Nature Communications

Figure 2: Morphological and molecular alterations in the spinal cords of PSL mice.

From: Glucocorticoid regulation of ATP release from spinal astrocytes underlies diurnal exacerbation of neuropathic mechanical allodynia

Figure 2

(a) Temporal profiles of Iba1-positive cells in the spinal cords of sham+PSL and ADX+PSL male mice. The dorsal horn areas are surrounded with dashed line. Scale bar, 100 μm. The right panel shows the quantification of the number of Iba1-positive cells (means±s.e.m.; n=5). Tukey–Kramer’s post hoc tests (PHT) shows that the number of Iba1-positive cells in the ipsilateral (Ipsi.) side of sham+PSL and ADX+PSL mice is significantly higher than that in the contralateral (Contra.) side (**P<0.01, *P<0.05), whereas no significant difference is noted in the number of cells between the two time points (F7,32=6.550, P<0.001; ANOVA). (b–g) Temporal profiles of mRNA levels for GCR/Nr3c1, Irf8, P2rx4, P2rx7, P2ry6 and P2ry12 in the spinal cords of sham+PSL and male ADX+PSL mice (means±s.e.m.; n=5–6). **P<0.01, *P<0.05 significantly different from contralateral sides in the corresponding groups (F7,36=13.307, P<0.001 for Irf8, F7,36=7.586, P<0.001 for P2ry6, F7,36=9.544, P<0.001 for P2ry12; ANOVA with Tukey–Kramer’s PHT).

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