Figure 1: NRKs are rate-limiting for NR-driven NAD+ synthesis.
From: NRK1 controls nicotinamide mononucleotide and nicotinamide riboside metabolism in mammalian cells
(a,b) NIH/3T3 cells were transfected with 4 μg of Flag-tagged WT and catalytically inactive version of NRK1 or NRK2 overexpressing vector. Nmrk1 and Nmrk2 mRNA (a) and NRK1/NRK2 protein (b) expression. (c) NAD+ measurement in NR-treated NIH/3T3 cells overexpressing NRK1 or NRK2. Results shown are mean±s.e.m.; EV, empty vector; *P<0.05 versus ctrl by two-sided unpaired T-test (n=3).
