Figure 6: Extracellular conversion of NMN to NR by hepatocytes.
From: NRK1 controls nicotinamide mononucleotide and nicotinamide riboside metabolism in mammalian cells
(a) Primary hepatocytes were isolated from 10 to 15 weeks old WT mice. Cells were treated with 2 μM FK866 and ±2 mM CMP for 1 h. Then, 0.5 mM NR or NMN was added and NAD+ was measured after 2 h. (b,c) WT (b) and NRK1KO (c) hepatocytes were treated with [2H]-[13C]-double labelled NR or NMN. Intracellular enrichment of labelled compounds. Results shown are mean±s.e.m., **P<0.01 versus ctrl by two-sided unpaired T-test (n=3).
