Figure 2: T_H1 and T_H2 differentiation in Irf8^–/– CD4⁺ T cells.

Naive CD4⁺ T cells of WT or Irf8^–/– mice were differentiated under T_H1 or T_H2 polarizing conditions for 4 days. Cells were then re-stimulated with PMA/ionomycin for 5 h and stained for intracellular IFN-γ as a T_H1 marker (a) and IL-4 as a T_H2 marker (b) by flow cytometry. Data are from one experiment representative of three independent experiments. Error bars, s.d. The cells prepared in a and b were re-stimulated with PMA/ionomycin for 12 h and the supernatants were analysed for IFN-γ and IL-4 by ELISA (c). Data are from one experiment representative of three independent experiments. Error bars, s.d. Naive CD4⁺ T cells from spleens and lymph nodes of Lck-Cre⁺Irf8^wt/wt and Lck-Cre⁺Irf8^fl/fl mice were prepared and the cells were differentiated under T_H0 and T_H17 polarizing conditions for 3 days. [³H]-Thymidine was added during the last 8 h of culture. Then the cells were collected and were counted with a beta-counter (d). Data are from one experiment representative of two independent experiments. Error bars, s.d.