Figure 3: Relative stability of dendritic spine density in the non-deprived adult visual cortex. | Nature Communications

Figure 3: Relative stability of dendritic spine density in the non-deprived adult visual cortex.

From: Recovery from chronic monocular deprivation following reactivation of thalamocortical plasticity by dark exposure

Figure 3

(a) Experimental timeline. (b) Representative DiI-labelled basolateral dendrites of pyramidal neurons in non-deprived binocular visual cortex from adult with binocular vision (Control), chronic monocular deprivation (Nondep), chronic monocular deprivation plus dark exposure (DE) and chronic monocular deprivation plus dark exposure and reverse deprivation (DE-RD). In each case, the dendritic segment shown is 75–100 μm from the cell body. Scale bar, 2.5 μm. (c) No significant differences in spine density in any dendritic segments following chronic monocular deprivation or dark exposure. Dark exposure and reverse deprivation induce a reduction in dendritic spine density in all dendritic segments (average spines/micron across 25 micron segments±s.e.m.; one-way ANOVAs: 0–25 μm F(3,69)=3.4272, P=0.0220; 25–50 μm F(3,69)=3.5132, P=0.0199; 50–75 μm F(3,68)=5.6344, P=0.0017; 75–100 μm: F(3,61)=9.7476, P<0.0001, *P<0.05 versus control, Tukey–Kramer honestly significant difference post hoc; n (neurons, subjects): Control (19,8), Nondep (24,9), DE (20,8), DE-RD (16,6).

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