Figure 4: Prox1 acts by activating NFAT signalling. | Nature Communications

Figure 4: Prox1 acts by activating NFAT signalling.

From: The transcription factor Prox1 is essential for satellite cell differentiation and muscle fibre-type regulation

Figure 4

(a) Heat maps from GSEA analysis showing that Prox1 overexpression induces the expression of genes known to be upregulated by calcineurin/NFAT signalling (red for upregulation compared with control) and decreases the expression of majority of genes downregulated by calcineurin/NFAT signalling (blue for downregulation compared with control). (b) qPCR analysis of regulator of calcineurin-1 (Rcan1) in Prox1 transduced TA muscles and Prox1 deficient soleus muscles. (c) NFAT-luciferase reporter assay in C2C12 cells transduced with retroviral (RV) Prox1 or GFP. (d) Nfatc1 staining in C2C12 cells overexpressing Prox1. (e) Nfatc1–3 mRNA expression in wild-type and Prox1 deleted soleus muscles Data is presented as mean±s.e.m. n=3+3 in a,c, n=5+5 and 4+4 in b, n=3+3 in d (repeated three times). Student’s two-tailed unpaired t-test, *P<0.05, ***P<0.001. Scale bar, 50 μm.

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