Figure 5: Lineage-tracing experiment of Prox1 expressing satellite cells. | Nature Communications

Figure 5: Lineage-tracing experiment of Prox1 expressing satellite cells.

From: The transcription factor Prox1 is essential for satellite cell differentiation and muscle fibre-type regulation

Figure 5

(a) HE staining of cardiotoxin-treated (CTX) and PBS-injected muscles showing the degeneration and regeneration processes 1, 4 and 22 days after CTX-injection. (b) Prox1 staining and TdTomato fluorescence 22 days after CTX-injection. Note several Prox1 negative red fibres, indicating that the Prox1 expressing satellite cells have differentiated into Prox1 negative muscle fibres. No red muscle fibres were found in the PBS-treated muscles. Arrows point to Prox1 and tdTomato-positive SCs. (c) The regenerated tdTomato positive muscle fibres originating from Prox1-positive SCs were MyHC I negative in fast tibialis anterior muscle. (d) TdTomato-positivity obtained with two tamoxifen injections; one injection was used in the experiments of a–c. Note that most of the regenerated fibres are red. Scale bars, 100 μm.

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