Figure 7: Bidirectional regulation between Prox1 and Notch1 in myoblasts. | Nature Communications

Figure 7: Bidirectional regulation between Prox1 and Notch1 in myoblasts.

From: The transcription factor Prox1 is essential for satellite cell differentiation and muscle fibre-type regulation

Figure 7

(a) Notch luciferase activity in C2C12 cells transduced with lentiviral shScr or shProx1, and later transfected with CSL- or Notch1-luciferase reporter. (b) Effect of overexpression of WT Prox1 and the Prox1 mutant lacking the DNA-binding domain (Prox1 ΔHP) on CSL- and Notch1-luciferase activities. (c) Effect of Prox1 overexpression on Notch-related genes in vivo in TA muscle. (d) Effect of adenoviral Notch1 overexpression on Prox1, CyclinD1, MyoD and Rcan1 mRNAs in human primary myoblasts. Nrarp was used to indicate Notch pathway activation. (e) Effect of Hey1 and Hey2 silencing on Notch1-induced suppression of Prox1 expression in human myoblasts. Data is presented as mean±s.e.m., n=4+4 biological replicates in a and b and 3+3 in d,e (all experiments were repeated two to three times), n=5+5 in c. Student’s two-tailed unpaired t-test, *P<0.05, **P<0.01, ***P<0.001.

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