Figure 2: Directional redistribution of vinculin within a podosome cluster. | Nature Communications

Figure 2: Directional redistribution of vinculin within a podosome cluster.

From: Actomyosin-dependent dynamic spatial patterns of cytoskeletal components drive mesoscale podosome organization

Figure 2

(a) DCs were transfected with tdEos-Vinculin and seeded in a glass bottom dish. Imaging of the green (non-photoswitched) and red (photoswitched) variants was performed by confocal microscopy. Photoswitching was induced by 405 nm laser short exposure in the circular area indicated by the dotted line. Redistribution of photoswitched proteins was measured in circular regions (b) and slices (c) and plotted as red to green ratio for each region (b). In c the red to green ratio was normalized to the maximum value. The colour of the plot lines corresponds with the regions indicated in the images above the plot. Scale bar, 10 μm.

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