Figure 2: Protein Cys bioconjugation with carbonylacrylic reagent 2c. | Nature Communications

Figure 2: Protein Cys bioconjugation with carbonylacrylic reagent 2c.

From: Stoichiometric and irreversible cysteine-selective protein modification using carbonylacrylic reagents

Figure 2

(a) Schematic of the reaction of Cys-tagged proteins with carbonylacrylic reagents 2a–d. (b–d) Electrospray ionization–MS spectra of conjugates (b) Annexin V-2c, (c) C2Am-2c and (d) Albumin-2c. (e) MS/MS spectrum of the m/z 526.75 doubly charged ion of the tryptic peptide VPYCELGGK, containing the modification at the Cys residue of C2Am-2c. The generated C-terminal y fragment ions (y6–y8) are consistent with the mass of the modification. (f) The albumin-2c conjugate remains intact when exposed to exogenous (GSH) thiols and in plasma as assessed by LC–MS. See Supplementary Fig. 21. RT, room temperature; DMF, dimethylformamide.

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