Table 1 Cys-bioconjugation on proteins.

From: Stoichiometric and irreversible cysteine-selective protein modification using carbonylacrylic reagents

Cys-protein (10 μM)

2

Equivalent

Temperature (°C)

Time (h)

Conversion (%)

Annexin V

2a

25

25

27

30†

Annexin V

2b

25

25

27

30†

Annexin V

2c

25

25

27

60

Annexin V

2d

25

25

27

0

Annexin V

2c

50

37

1

>95

C2Am

2c

25

25

1

>95

Albumin

2c

1

25

2

>95

  1. Identification of an efficient carbonylacrylic reagent and reaction optimization for site-selective Cys modification on proteins.*
  2. *General bioconjugation conditions: protein (10 μM) in TrisHCl (pH 8.0, 20 mM), carbonylacrylic derivative 2a–d (1–50 equiv.) dissolved in dimethylformamide (1–10%) were reacted in an Eppendorf tube for the indicated time.
  3. †Hydrolysis of the ester was observed by LC–MS. See Supplementary Methods and Supplementary Figs 8–12 for full details. The bold numbers are the compound numbers.
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