Figure 2: ABCD1 regulates the induction of CH25H expression in a VLCFA-dependent manner. | Nature Communications

Figure 2: ABCD1 regulates the induction of CH25H expression in a VLCFA-dependent manner.

From: 25-hydroxycholesterol contributes to cerebral inflammation of X-linked adrenoleukodystrophy through activation of the NLRP3 inflammasome

Figure 2

(a,b) Cellular levels of VLCFA (a) and mRNA levels of CH25H (b) in control fibroblasts untransfected or transfected with scrambled- or ABCD1-targeting siRNA (ABCD1 KD) and in CCALD1 fibroblasts. (n=6) (c,d) Cellular levels of VLCFA (c) and mRNA levels of CH25H (d) in CCALD1 fibroblasts infected with lentiviral particles expressing empty vector or ABCD1 gene. (n=5) (e) Relative mRNA expression of Cyp46a1, Ch25h and Cyp27a1 in the brain homogenates of WT or Abcd1-deficient mice. (n=6). (f,g) Relative mRNA levels of CH25H in control- or two CCALD-fibroblasts (f) and in CCALD-OPCs (g) treated with vehicle (0.05% DMSO in PBS) or VLCFA at indicated concentration for 3 h. (f, n=6; g, n=5) For all panels, *P<0.05, **P<0.01, and ***P<0.001. NS, not significant.

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